Acute toxicity evaluation of Tricyclozole 75% WP on earthworms Eisenia fetida

Abstract

Earthworms (Eisenia foetida) were exposed to Tricyclozole 75%  WP pesticides in an acute earthworm toxicity test as per OECD guideline 207. Four replicates of ten clitellated adult earthworms each were exposed to the  concentrations of 31.25, 62.5, 125, 250, 500, and 1000 mg/kg dry soil. The control artificial soil with acetone and quartz sand had four replicates, with ten earthworms in each replicate. Earthworms were assessed for behavioural effects at 0, 7, and 14 days of exposure, mortality after 7 and 14  days of exposure, and the  earthworm biomass was assessed on day 0 and on day 14. The results of the study revealed acute toxicity of Tricyclozole 75% WP to the earthworm Eisenia fetida in artificial soil.