Genetic peculiarities of free radical oxidation of lipids and proteins in the semen of breeding boars

  • Svitlana Polishchuk
  • Svitlana Tsekhmistrenko
  • Vitalii Polishchuk
  • Oksana Tsekhmistrenko
  • Nataliia Ponomarenko
  • Olexandra Seleznyova
Keywords: breeding boars, semen, sperm, peroxide oxidation of lipids, enzymes of antioxidant system, oxidation modification of proteins, medium mass molecules


Complex research into biochemical aspects of the functioning of the antioxidant system for the protection of sperm of the breeding boars has established that the intensity of the flow of peroxide oxidation of lipids in germ cells is much higher than in the extracellular space. Reactions of free radical peroxide oxidation of proteins and lipids in the semen of healthy breeding boars are characterized by a stable level of activity that is necessary for the normal course of implementation of processes of the reproductive function. All components of the antioxidant system stay in mutual compensatory ratios under physiological conditions. As a rule, reducing the concentration or activity of some antioxidant enzymes leads to corresponding changes in others. The main antioxidants of the germ cell genomes are superoxide dismutase and ceruloplasmin. Catalase is a key enzyme that neutralizes H2O2 in the semen fluid of breeding boars. The content of total proteins in the semen of the synthetic line SS23 animals is greater (p < 0.05) than in the breeding boars of the large white breed. The processes of the oxidative modification of proteins in the animals body of the synthetic line runs more intensely, as it is evidenced by the higher content of the aldehyde and ketodinitrophenyl hydrazones of the main and neutral character in the sperm cytoplasm.