Immobilization of Enterobacter aerogenes E13 strain culture medium, distinguished by its lipase activity, in the ethylcellulose microcapsules

Abstract

Lipases – enzymes with hydrolytic activity widely applied in many fields of the industry, used to hydrolyze certain substrates and synthesize organic compounds. One of the main methods of enzyme stabilization is that of their immobilization using various carriers. For economic purposes, microorganism culture media with catalytic activity can be immobilized by skipping the purification. The way to immobilize lipase producing bacterium Enterobacter aerogenes E13 strain culture medium in ethylcellulose microcapsules by microcapsulation has been applied in this work. The influence of the microcapsulation process conditions towards the specific lipasic activity and immobilization activity of immobilized preparation has been examined. The properties of immobilized preparation have been determined: pH and temperature optimums, stability and physical indexes. The rapeseed oil has been hydrolyzed with this preparation and peresterified with methanol. Keywords: lipase, Enterobacter aerogenes, microcapsulation, hydrolysis, peresterification