Performance characteristics of single-stage biohythane production by immobilized anaerobic bacteria

Doan Thanh Ta, Chiu Yue Lin, Chen Yeon Chu, Thi Minh Ngoc Ta


Biohythane produced via dark fermentation is much greener than hythane that is generated using natural gas. Biohythane production using a single-stage system has potential to increase the economic viability since it requires fewer controls than a two-stage system that has individual acidogenic and methanogenic reactors. This single-stage system is an innovative method in producing biohythane. The present work investigated the performance of a mesophilic single-stage system with a batch mode operation to generate biohythane. The reactor was seeded with hydrogenic and methanogenic bacteria (HB and MB), which were entrapped in κ-carrageenan/ gelatin beads (2%/2% w/w) using the dripping method. The energy yield of 0.41 to 1.48 kJ g–1 glucose and the hydrogen content in biohythane (H2/(H2 + CH4)) of 0.35 to 0.69 were obtained. These results indicate that different biohythane compositions would be obtained by regulating the HB/MB bacteria concentration ratio, substrate concentration and cultivation pH. Moreover, a comparison of two-stages and single-stage systems as well as the challenges were also elucidated.


anaerobic digestion; biohythane; cell immobilization; κ-carrageenan; gelatin

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ISSN 0235-7208 (Print)
ISSN 1822-8836 (Online)