Effect of water extraction of true hemp seeds (Cannabis sativa L.) on legume plants in the organogenesis stage I

Abstract

This article presents the research focusing on the effect of water extraction of true hemp seeds (Cannabis sativa L.) (50% concentration) of lupines (Lupinus augustifolius), beans (Pisum sativum) and peas (Phaseolus vulgaris) in the organogenesis stage I (seed germination, length of roots, height of shoots, ratio of root/shoot length). The experiment was conducted at the Laboratory of the Institute of Biology and Plant Biotechnology, Aleksandras Stulginskis University in 2017. The control was distilled water. The experiment was repeated three times. It has been determined that the water extraction of the true hemp seeds had no considerable effect on the germination of legume plants. The extract did not significantly stimulate the root and shoot elongation of the lupines ‘Max’ and ‘Snaigiai’. The extract considerably shortened the root length of ‘Baltija’ beans by 1.59 times and the shoot length by 1.27 times. The hemp seeds shortened the root and shoot length of ‘Kiblukai’ peas by 1.45 and 1.94, respectively. The effect of the water extracts of true hemp seeds on the legume plants in the organogenesis stage I depended on their genotype.