Factors affecting Miscanthus × giganteus adventitious shoots formation in vitro
Keywords: adventitious shoots, basal nutrition medium, growth regulators, Miscanthus × giganteus, subculture
AbstractThe research was carried out at the Laboratory of Agrobiotechnology of the Institute of Biology and Plant Biotechnology, Faculty of Agronomy of Vytautas Magnus University (till 2019 Aleksandras Stulginskis University). The effect of basal nutrition medium, subculture and growth regulators on the adventitious shoots formation from isolated explants of Miscanthus × giganteus was analysed. The apical meristem of in vitro developed adventitious shoots of Miscanthus × giganteus was cultivated in the Woody Plant Medium (WPM) and Murashige and Skoog (MS) nutrition medium supplemented with different 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) combinations. It has been determined that the formation frequency of adventitious shoots on the WPM nutrition medium was from 5.0 to 18.5% (after the first subculture) and from 19.5 to 38.3% (after the second subculture) higher in comparison with the MS nutrition medium supplemented with the same combinations of growth regulators. It has been established that on the WPM nutrition medium the formation frequency of adventitious shoots after the second subculture was from 13.3 to 18.5% higher in comparison with the first subculture, while on the MS nutrition medium the effect of subculture was not significant. The most intensive frequency of shoots formation (48.8%) and the highest shoot number per explant (24.1 units) have been obtained in the WPM medium supplemented with 3.0 mg l–1 BAP + 0.4 mg l–1 IBA + 0.01 mg l–1 IAA after the second subculture.