Membrane-modifying properties of hydrated fullerene C60 in combination with Spirulina platensis cell culture

Abstract

The study is devoted to the development of new promising nanocomponent compositions with membrane-modulating properties. The effect of a solution of hydrated fullerene C₆₀ and a cell suspension of blue-green algae Spirulina platensis on the activity of free radical lipid peroxidation and oxidative phosphorylation in mitochondria of rat liver was studied. Fullerene C₆₀ and suspension of Spirulina platensis cells were administered to Wistar rats for 30 days, once a day, intragastrically, both jointly and singly. The rate of oxygen consumption of phosphorylating and non-phosphorylating mitochondria (in V₃ and V₄ states), the rate and efficiency of ADP phosphorylation (ADP/Δt and ADP/O), respiratory control coefficient (RC) were changed in rat liver mitochondria. In the same samples, the concentration of diene, triene, oxodiene, and tetraene fatty acids was measured. The results of our studies have shown that the solution of hydrated fullerene C₆₀ serves as a structural modifier of the lipid bilayer of cell membranes, the effect of which can be corrected by using an antioxidant component such as a suspension of Spirulina platensis cells.