Home-made silver ion solid-phase extraction system for the analysis of trans fatty acids: comparison with commercial Discovery Ag-Ion SPE

  • Vilius Poškus
  • Vida Vičkačkaitė
  • Julita Dargytė
  • Gintautas Brimas
Keywords: Ag -SPE, trans fatty acids, human adipose


A home-made silica-based silver ion solid-phase extraction (Ag+-SPE) system for the fractionation and subsequent gas chromatographic analysis of trans fatty acids in human adipose tissue is developed and examined. Analytical characteristics of the home-made Ag+-SPE column were compared with those of the commercial Discovery Ag-Ion SPE column and it was demonstrated that the both columns can be applied for the fractionation of fatty acid methyl esters.
Analytical Chemistry