Optimization of matrix solid-phase dispersion extraction for the chromatographic analysis of flavones in cranberries

Abstract

A matrix solid-phase dispersion (MSPD) extraction method was applied for three flavones in cranberry (Vaccinium macrocarpon Ait.) matrix for the first time. The optimized MSPD procedure required a small amount of the sample (0.25 g), C18 bonded silica gel (1.0 g) as a dispersant solid-phase sorbent and small volumes of methanol using its aqueous solutions (10 mL) as eluting solvents. Identification and quantification of myricetin, quercetin and kaempferol were performed by HPLC-DAD-MS. The limit of detection varies from 0.04 μg/g to 0.08 μg/g with linear regression coefficients (r2 > 0.999) for each compound. The recoveries of the analytes ranging from 59% to 111% with relative standard deviations less than 9.5% were checked using spiked freezedried cranberry samples. The MSPD method is simple, rapid and efficient for the extraction of flavones from cranberry samples.